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KMID : 1140120060110030192
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Cancer Prevention Research
2006 Volume.11 No. 3 p.192 ~ p.198
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Bax Inhibitor-1 Suppresses Hydrogen Peroxide-induced Apoptosis by Regulating Intracellular ROS Level
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Kim Su-Jeong
Kim Jung-Hyun
Lee Eung-Ryoung
Jeong Hyo-Soon
Cho Ssang-Goo
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Abstract
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Bax Inhibitor-1 (BI-1) is an anti-apoptotic transmembrane protein that is related with Bcl-2 family proteins, but it does not belong to any known gene family of vertebrates. BI-1 seemed to be an ancient regulatory protein, as homologues of BI-1 exist in not only most eukaryotes but prokaryotes. Recent studies showed that overexpression of BI-1 were observed in several kinds of cancers and led to suppression of the apoptotic cell death. BI-1 was found to have anti-apoptotic and oncogenic effects, but the exact molecular mechanism for the effects was not completely understood. Here, in order to
investigate the relationship between the anti-apoptotic functions of BI-1 and reactive oxygen species (ROS) production, we studied the effect of BI-1 overexpression on the hydrogen peroxide-induced apoptosis. Cell death was induced by hydrogen peroxide treatment in a dose-dependent manner and found to be suppressed by stable expression of human BI-1. From DAPI-staining nuclear fragmentation analysis, stable expression of human BI-1 was assessed to protect the cells against hydrogen peroxide-induced apoptosis. Moreover, BI-1 expression had effect on decreasing the intracellular ROS level induced by hydrogen peroxide-caused oxidative stress. These results indicate that BI-1 could suppress hydrogen
peroxide-induced apoptosis by regulating intracellular ROS level. (Cancer Prev Res 11, 192-198, 2006)
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KEYWORD
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Bax Inhibitor-1, Apoptosis, Reactive oxygen species, Hydrogen peroxide
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